Search results for "Imidazolidinyl urea"

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Flow-cytometric screening for the modulation of receptor-mediated endocytosis in human dendritic cells: implications for the development of an in vit…

1997

The aim of this study was to explore the usefulness of human blood dendritic cells (DC) in the development of an in vitro model for predictive testing of contact sensitizers. A method was established to monitor the influence of chemicals on the intracellular targeting of antibody-crosslinked MHC class II molecules after their uptake by human DC. Using a three-colour flow-cytometric technique, freshly prepared DC were distinguished from other MHC class II-bearing cell types such as B-cells and monocytes in unseparated mononuclear cell suspensions of healthy volunteers. The assay is based on the pH-sensitivity of internalized fluorescein-coupled MHC class II specific antibodies. Quenching of …

Intracellular Fluidmedia_common.quotation_subjectImmunologyFluorescencechemistry.chemical_compoundPredictive Value of TestsIn vivoMHC class IDithranolmedicineHumansImmunology and AllergyReceptors ImmunologicInternalizationmedia_commonMHC class IIbiologyChemistryAntibodies MonoclonalDendritic CellsHLA-DR AntigensDendritic cellHydrogen-Ion ConcentrationFlow CytometryFluoresceinsEndocytosisIn vitroImidazolidinyl ureaImmunologybiology.proteinBiophysicsHaptensmedicine.drugJournal of Immunological Methods
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